Rapid Quantitative PCR Assay for the Detection of the Three Vaginal Pathogens <i>Candida</i>, <i>Gardnerella</i> and <i>Atopobium</i> as well as the Commensal <i>Lactobacillus</i> Genera

The vaginal microbiota balance is quite fragile and susceptible to the development of vaginosis candidiasis. current diagnostic method for bacterial relies on evaluation different morphotypes using Nugent score. This only partially in correlation with a DNA sequencing-based or Amsel criteria used by clinicians, suggesting need new molecular approaches dedicated diagnosis BV. objective this study was develop validate quantitative polymerase chain reaction (qPCR) assay specific rapid detection three pathogens, i.e. Candida, Gardnerella Atopobium commensal Lactobacillus genera. For purpose, four oligonucleotide primer pairs were designed tested obtain optimal amplification target qPCR also non-target genera human DNA. primers allowed organisms vitro clinical samples. effective specifically detect these samples as technique complementary It can be epidemiological studies understanding role pathogens follow their abundance disease processes such vulvovaginal candidiasis vaginosis.